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KMID : 0613819990090010029
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Journal of Life Science
1999 Volume.9 No. 1 p.29 ~ p.34
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Changes of RNA and Protein During Callus Induction and Plant Regeneration from Pefilla fruescens
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Abstract
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Cotyledon and hypocotyl explants of perilla were cultured on MS medium containing a combined concentration of BA(0.5, 10 and 1,5mg/l) and NAA(0.1, 0.5 and 2.0mg/l) in order to regenerate the explant and induce the callus. the best regeneration of the explant and induction of the callus were observed in a combined concentration of 0.5mg/l of BA and 0.5mg/l NAA both in cotyledon and hypocotyl explants. In cotyledon explants, rooting was achieved upon transferring shoots to MS medium containing 0.5mg/l of BA and 0.1mg/l of NAA. We also investigated the change of protein and RNA content on developmental stage of callus and plant regeneration of perilla. Protein content was increased but RNA content was decreased as the culture period increase. The banding pattern of polypeptide revealed that both 30KD and 45KD polypeptides were obvious in cotyledon obtained from pre-culture explants, but only 30KD polypeptide was further getting obvious as the culture period increases
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